Serologic responses to peptides of Anaplasma phagocytophilum and Borrelia burgdorferi in dogs infested with wild-caught Ixodes scapularis

摘要: Abstract Anaplasma phagocytophilum and Borrelia burgdorferi are both transmitted by Ixodes spp. associated with clinical illness in some infected dogs. This study evaluated canine antibody responses to the A. p44 peptides APH-1 APH-4 as well B. C6 peptide before after doxycycline treatment. A total of eight dogs were infested wild-caught I. scapularis for 1 week. Blood was collected prior tick attachment from Days 3–77 218–302 treatment beginning on Day 218. assayed DNA PCR assay. Sera assessed antibodies immunofluorescent (IFA) test ELISA. amplified blood all 7. Antibodies detected serum early 14 days exposure six had 3–7 days against APH-1. All seropositive 218 302. 6/8 21 days infestation. Among five that remained at 218, levels declined average 81% within 84 days initiating The results suggest may be more useful than detecting earlier course an infection. After administration, but not or decreased, suggesting a effect production.