Antibiotic assays principles and precautions.

摘要: Publisher Summary This chapter discusses the principles and precautions of various assays for antibiotics. The problems application, indicates reasons assaying, considers general topic relation between structure activity. Two kinds are usually referred: dilution diffusion. In former, antibiotic is presented to test organism at one concentration. concentration uniform throughout medium. medium may be liquid as in tube methods or solidified by agar a plate method. Activity measured an end—as serial tubes plates—or graded response, diffusion turbidimetric methods. Three use—namely, dilution, tubes, photometric assays. Some sort all-or-none response obtained with first two types. third represents high precision which concentrations drug.