Effects of Rosa laevigata Michx. extract on reactive oxygen species production and mitochondrial membrane potential in lens epithelial cells cultured under high glucose.
作者： Xiangyu Luo , Weimin Luo , Yuefeng Liu , Zhang Yong , Xiaodong Zhong
DOI:
关键词: Matrix metalloproteinase 、 Cell 、 Western blot 、 Protein kinase B 、 Molecular biology 、 Reactive oxygen species 、 Membrane potential 、 PI3K/AKT/mTOR pathway 、 Chromosomal translocation 、 Biology
摘要: This study was to investigate the effects of Rosa laevigata Michx. (RLM) extract on reactive oxygen species (ROS) production and mitochondrial membrane potential (MMP) in lens epithelial cells (LECs) cultured under high glucose. SRA01/04 cell models diabetic cataract were established by glucose culture, these administrated with RLM extract. Levels ROS MMP detected fluorescent probes. HO-1 expression level, Akt phosphorylation, Nrf2 translocation Western blot. treatment could exert protective condition, decreasing elevating cells. blot analysis indicated that level significantly elevated after treatment. Moreover, when interfered siRNA, levels diminished, indicating induction necessary for function RLM. Furthermore, inducing mediated PI3K/Akt Nrf2/ARE pathways. The would be abolished either pathway inhibited pharmacological manipulation or siRNA silencing. inhibits elevates MMP, through expression, LECs condition. are Our findings provide theoretic basis experimental evidence application cataract.
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