A Fast SSP-PCR Method for Genotyping the ATP-Binding Cassette Subfamily B Member 1 Gene C3435T and G2677T Polymorphisms in Chinese Transplant Recipients:

摘要: Aim P-glycoprotein, the product of ATP-binding cassette subfamily B member 1 (ABCB1) gene (or so-called multidrug resistance gene), is an ATP-driven efflux pump contributing to pharmacokinetics as well drugs that are P-glycoprotein substrates, such tacrolimus. This paper describes development a new method for detection 3435C/T and 2677G/T/A single nucleotide polymorphisms ABCB1 gene. The simple sequence-specific primer polymerase chain reaction (SSP-PCR). Methods 158 Chinese health checkup examinees 214 transplant recipients were included in study. Genomic DNA was extracted from peripheral blood amplified with SSP-PCR detect mutations ABCB1. condition optimized, PCR results compared those sequencing. Results In optimized condition, two could be clearly distinguished after one-step electrophoresis. genotypes subjects scanned, allele-specific bands successfully by SSP-PCR, which full accordance Conclusion As fast, inexpensive genotyping tool, would practicable large clinical studies on interindividual pharmacokinetics.