G3BP1 contributes to tumor metastasis via upregulation of Slug expression in hepatocellular carcinoma.
作者： Jingde Chen , Yandong Li , Ning Dou , Yong Gao , Shijun Yu
DOI:
关键词: Cancer research 、 Cell migration 、 RNA interference 、 Biology 、 Gene knockdown 、 Downregulation and upregulation 、 Gene silencing 、 Cell Migration Inhibition 、 Slug 、 Metastasis
摘要: RasGAP SH3-domain-Binding Protein 1 (G3BP1) has been implicated in cell growth, migration, and metastasis of some cancers, yet its function hepatocellular carcinoma (HCC) remains to be explored. In the present study, we reported that G3BP1 was upregulated HCC tissues compared with adjacent non-cancerous liver both mRNA protein levels, high expression significantly correlated poor prognosis patients. Functional analyses demonstrated forced cells promoted silenced by RNA interference caused opposite effects. Moreover, knockdown attenuated distant capacity through tail vein injection approach nude mice model. At molecular mechanism, found decreased Slug expression, increased epithelial marker E-cadherin. Overexpression could restore phenotype silencing induced migration inhibition. Together, our data establish as an oncogenic factor involved suggest might serve a novel predictor for patients' outcome.
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