HACKING THE CENTROMERE CHROMATIN CODE: dissecting the epigenetic regulation of centromere identity

摘要: 5 ABBREVIATIONS 7 CONTENTS 9 INTRODUCTION 12 A CHROMOSOMES, MITOSIS AND KINETOCHORES: AN OVERVIEW A.1 The Packaging of DNA in Chromosomes A.2 Stages Mitosis 13 A.3 Kinetochore 16 B EPIGENETICS REGULATION OF CHROMATIN ARCHITECTURE 20 B.1 Basic Concept “Epigenetic” Regulation B.2 Histone Lysine Methylation 21 B.3 Transcription the Modulation Chromatin Architecture and Function 24 C THE CENTROMERE 28 C.1 Sequence Centromere Identity 29 Centromere-specific H3 Variant CENP-A 32 C.2 Network Constitutive Proteins forms Basis for Assembly 34 C.3 Maintenance Deposition 41 C.4 Domain 46 D HUMAN ARTIFICIAL CHROMOSOMES 52 D.1 Generation Biology Human Artificial D.2 Alphoid Chromosome 54 E AIMS THIS WORK 61 MATERIALS METHODS 64 F F.1 Bacterial Eukaryotic Cell Culture F.2 Buffers, Solutions Reagents F.3 Oligonucleotide primers 66 F.4 Antibodies 69 F.5 Commercial Kits 71 G MOLECULAR BIOLOGY TECHNIQUES 72 G.1 Preparation Plasmid