A possible involvement of TIF1 alpha and TIF1 beta in the epigenetic control of transcription by nuclear receptors.

摘要: Nuclear receptors (NRs) are ligand-inducible transcription factors that mediate complex effects on development, differentiation and homeostasis. They regulate the of their target genes through binding to cognate DNA sequences as homodimers or heterodimers. The molecular mechanisms underlying transcriptional activation by NRs still poorly understood, although intermediary (mediators) appear be involved in mediating transactivation functions NRs. TIF1 has been identified previously a protein interacts specifically with ligand domain several nuclear receptors, both yeast vitro. characteristics these interactions have led us suggest might mediator NR function AF-2. Using two-hybrid screening yeast, we now two TIF1-binding proteins, mHP1 alpha mMOD1, mouse homologues Drosophila heterochromatinic 1. bait second screening, isolated cDNAs encoding proteins also very likely chromatin structure function, well structurally functionally related (renamed alpha), which was named beta. Here discuss how members family control could exerted at level template.