The Fluorescent Staining of Bacteriophage Nucleic Acids

摘要: SUMMARY: The staining of viral nucleic acids with acridine orange and their subsequent examination under the fluorescence microscope permits determination type strandedness. Since existing procedures are complicated, modifications have been devised which simplify them avoid use microscope, an ordinary ultraviolet lamp being substituted. Also, it has found that certain post-staining treatments cause colour changes related to strandedness acid hence valuable confirmations normally used nuclease sensitivity tests. new were tested for a wide range specimens including double-stranded ribonucleic acid, but emphasis placed on identification bacteriophage acids.