Bone repair by periodontal ligament stem cellseeded nanohydroxyapatite-chitosan scaffold.

摘要: Background A nanohydroxyapatite-coated chitosan scaffold has been developed in recent years, but the effect of this composite on viability and differentiation periodontal ligament stem cells (PDLSCs) bone repair is still unknown. This study explored behavior PDLSCs a new genipin-chitosan conjunction (HGCCS) vitro as compared with an uncoated framework, evaluated PDLSC-seeded HGCCS vivo. Methods Human were cultured identified, seeded assessed by scanning electron microscopy, confocal laser MTT, alkaline phosphatase activity, quantitative real-time polymerase chain reaction at different time intervals. Moreover, scaffolds used rat calvarial defect model, formation was hematoxylin eosin staining 12 weeks postoperatively. Results clonogenic positive for STRO-1. They had capacity to undergo osteogenic adipogenic vitro. When HGCCS, exhibited significantly greater viability, upregulated bone-related markers, sialoprotein, osteopontin, osteocalcin extent framework. The use promoted repair. Conclusion demonstrates potential combined promising tool regeneration.