Method of determining zygosity by ligation and cleavage
作者： Sydney Brenner
DOI:
关键词: Sequencing by ligation 、 Ligation 、 Biochemistry 、 Nucleotide 、 DNA 、 Polymerase 、 Molecular biology 、 Polynucleotide 、 Nucleic acid sequence 、 Biology 、 Cleavage (embryo)
摘要: The invention provides a method of nucleic acid sequence analysis based on repeated cycles ligation to and cleavage probes at the terminus target polynucleotide. At each such cycle one or more terminal nucleotides are identified removed from end polynucleotide, that further can take place. is shortened by until nucleotide polynucleotide determined. obviates electrophoretic separation similarly sized DNA fragments eliminates difficulties associated with detection spatially overlapping bands in gel, like medium. need generate long single stranded templates polymerase.
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