Use of temperature-sensitive mutants to study gene expression during sporulation in Bacillus subtilis.

摘要: Two temperature-sensitive sporulation mutants have been characterized. One mutant, which is blocked at stage II, has a short period that occurs about the time when spore septum formed. Cells can escape block, if incubated for permissive temperature, but are prevented from doing so by inhibitors of transcription and translation; this suggests product defective gene protein messenger ribonucleic acid codes short-lived. The other mutant IV to V long starts during III precedes mutational defect phenotypically expressed. behavior in temperature-shift experiments synthesis commences before it assumes its physiological function.