Mass-sensing, multianalyte microarray immunoassay with imaging detection.

摘要: Miniaturization of ligand binding assays may reduce costs by decreasing reagent consumption, but it is less apparent that miniaturized can simultaneously exceed the sensitivity macroscopic techniques analyte "harvesting" to exploit total mass available in a sample. Capture reagents (avidin or antibodies) immobilized 200-microm diameter zones are shown substantially deplete from liquid sample during 1-3-h incubation, and result sense rather than its concentration. Detection as few 10(5) molecules per zone possible fluorescence imaging situ on solid phase using near-infrared dye label. Single multianalyte mass-sensing sandwich array IgG subclasses show specificity ELISA methods use 1/100 capture antibody required 96-well plate format.