Immunochemistry at interfaces.

摘要: The immunochemistry of antibody binding to solid-phase immobilized antigen is reviewed. Experimental data are compared with different theoretical models reaction mechanisms at solid-liquid interfaces. It was found that reactions the interface can become limited by diffusion rate due depletion reactants close surface, even though intrinsic bimolecular surface reaction-rate limited. forward constant decreases increasing concentration bound antibodies and when not be more than 1000-fold slower corresponding in a liquid solution. Possible explanations for this phenomenon discussed. dissociation slow process solid phases. antigen-antibody complexes formed practically irreversible. Some evidence presented which indicates stability these attractive lateral interactions between antibodies.