Determination by ellipsometry of the affinity of monoclonal antibodies
作者： Håken Nygren , Matti Kaartinen , Manne Stenberg
DOI: 10.1016/0022-1759(86)90169-9
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摘要: The reaction between monoclonal antibodies and surface-immobilised hapten was studied by ellipsometry, a method allowing absolute measurement of the surface concentration proteins. Monoclonal against 2-phenyloxazolone were used their affinity for antigen in solution determined calculations equilibrium constant from data obtained measuring fluorescence quenching due to antibody binding. binding rate dissociation complex measured ellipsometry. heterogeneous antigen-antibody Scatchard plot. found be higher than homogeneous factor which varied different antibodies.
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