Influence of Diffusional Limitations and Lateral Interactions on Antigen–Antibody Binding Kinetics

摘要: This chapter develops kinetic expressions for the binding of an antigen in solution by immobilized antibody and antigen. It also analyzes similarities dissimilarities different rate forms two types systems. In general, results are applicable to analyte–receptor reaction systems on biosensor as well other surfaces, such cellular surfaces. The dual-step expression surface exhibits a second-order dependence concentration close surface. is easily extended case where lateral interactions involved between antibody–antigen complexes. first-order both available binding. has been found that conclusions apply time variant adsorption coefficient far concerned time-dependent coefficient.