Accumulation of Three Important Bioactive Compounds in Different Plant Parts of Withania somnifera and its Determination by the LC–ESI-MS-MS (MRM) Method
作者： Narendra A. Gajbhiye , Jayanti Makasana , Satyanshu Kumar
关键词: Selected reaction monitoring 、 Chemistry 、 Chromatography 、 Acetic acid 、 Mass spectrometry 、 Electrospray ionization 、 Withania somnifera 、 Tandem mass spectrometry 、 Analyte 、 Acetonitrile
摘要: A comprehensive experiment was conducted to study the accumulation pattern and determination of three important bioactive compounds namely withaferin-A (WA), 12-deoxywithastramonolide (WO) withanolide-A (WD) its by liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS-MS) method in root, stem, fruits leaves Withania somnifera. rapid sensitive LC-ESI-MS-MS developed validated for these compounds, having same molecular weight. The multiple reaction monitoring established two transitions each analyte intense transition used quantification. Separation analytes achieved within a run time 5 min on an RP-18 column using mobile phase consisting acetonitrile 0.1% acetic acid water isocratic condition. as per ICH guidelines. found be suitable identification quantification WA, WO WD different plant parts such roots, stems, W. WA highest samples (8.84 ± 0.37 mg/g) it 2.23, 5.85 27.26 times higher than concentration fruits, stems respectively. contents were (0.44 0.016 0.72 mg/g, respectively) root.
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