In vitro regeneration of Aristolochia tagala and production of artificial seeds
作者： M. Remya , V. Bai , V. N. Mutharaian
DOI: 10.1007/S10535-012-0280-2
关键词: Botany 、 Polyphenol oxidase 、 Organogenesis 、 Shoot 、 Kinetin 、 Aristolochia tagala 、 Callus 、 Biology 、 Murashige and Skoog medium 、 Auxin
摘要: Protocols for in vitro plant multiplication from somatic tissues and production of artificial seeds through encapsulation nodes were developed Aristolochia tagala Cham., a rare valuable medicinal plant, as measure conservation prerequisite genetic transformation procedure. A maximum number adventitious shoots regenerated leaf-derived callus on Murashige Skoog (MS) medium containing 6-benzylaminopurine (BAP; 2 μM), α-naphthaleneacetic acid (NAA; 0.5 phloroglucinol (PG; 10μM). Nodes collected established shoot cultures encapsulated 3 % (m/v) sodium alginate 1 calcium chloride. Multiple successfully the cultured MS supplemented with μM BAP kinetin (KIN). Regenerated rooted acclimated to greenhouse conditions. Since roots A. are primarily used traditional medicine, protocol regenerating directly leaf derived was also developed. Maximum root length obtained when KIN (1 indole acetic (IAA; NAA (0.1 PG (10 μM). Biochemical parameters studied calli grown without establish correlation between these morphogenesis. An increment antioxidant enzymes (peroxidase catalase) metabolites (sugars proteins), decrease amount polyphenol oxidase observed which presence PG.
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