The peptide product of a 5' leader cistron in the beta 2 adrenergic receptor mRNA inhibits receptor synthesis.
作者： A.L. Parola , B.K. Kobilka
DOI: 10.1016/S0021-9258(17)41806-0
关键词: 5-HT5A receptor 、 Open reading frame 、 Beta-2 adrenergic receptor 、 Molecular biology 、 Receptor 、 Biology 、 Signal peptide 、 Fusion protein 、 Receptor expression 、 Cistron
摘要: The 5' leader region of mammalian beta 2 adrenergic receptor messenger RNAs (mRNA) have a short open reading frame (sORF) preceding the cistron. Mutational inactivation sORF start codon increased expression and translation 1.9-fold from genes transfected into COS-7 cells. also synthesis 2.4-fold in cell-free system that synthesizes functional vitro. Translational initiation at was demonstrated both vitro cells using an epitope-tagged fusion protein. Using protein as reporter for shows mutations which increase decrease translation. Mutation analysis peptide coding sequences suggests itself inhibits expression. Consistent with this hypothesis, synthetic corresponding to encoded by potently Our results suggest nonoverlapping cistron mRNA is translated resulting
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