Fluorescent cell labeling for in vivo and in vitro cell tracking.

作者: Paul Karl Horan , Meryle J. Melnicoff , Bruce D. Jensen , Sue E. Slezak

DOI: 10.1016/S0091-679X(08)60547-6

关键词: Flow cytometryBiologyBiochemistryCellCell membraneReceptorIntracellularEndocytosisCell migrationIn vivo

摘要: Publisher Summary In cell biology, a valuable goal is to arrive at better understanding of how specific types perform in vivo. This chapter discusses the molecular nature intercellular recognition processes, an example which lymphocyte T-cell receptor. While these receptors are important, they functional only close range. The ability track vivo migration labeled cells provides insight into differentiation processes tissues, such as thymus or bone marrow. Cell-tracking technology applied tumor determine whether tumor-infiltrating lymphocytes migrate metastatic sites. study cellular atherosclerotic plaque would be extreme value pathophysiology coronary disease. Information obtained by studying and out organ prior during rejection process after transplants. describes use PKH2 linker kits. label aliphatic fluorescent chromophore. staining methodology allows for labeling lipids membrane rather than surface protein labeling.

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