Male Donor-derived Cells in the Brains of Female Sex-mismatched Bone Marrow Transplant Recipients: A Y-Chromosome Specific In situ Hybridization Study

摘要: In five female bone marrow transplant (BMT) recipients of sex-mismatched donor marrow, Y-chromosome specific in situ hybridization was performed on formalin-fixed, paraffin-embedded sections the medulla to detect male marrow-derived cells. Y-chromosome-bearing cells (Y-cells), thereby donor-derived, were matched with leukocyte common antigen (LCA)-reactive adjacent immunostained anti-LCA antibody. Y-cells included mononuclear leukocytes (MNL) within vessel lumen and infiltrating perivascular space parenchyma, "perivascular cells." We have, therefore, concluded that MNL, though limited number, do enter normal-appearing brain can transform cells" human BMT recipients. It remains, however, be confirmed whether MNL entering normal adult CNS parenchyma ramified microglia.