Comparison of p53 mutations in patients with localized osteosarcoma and metastatic osteosarcoma.

摘要: BACKGROUND In some malignancies, p53 mutations are associated with tumor progression. To address the role of in development and progression osteosarcoma, authors analyzed specimens from 247 patients primary localized osteosarcomas 25 that were metastatic at time diagnosis. The group included 27 matched biopsy-resection 21 biopsy-metastasis paired specimens. METHODS The examined nature location (exons 4–10) by polymerase chain reaction–single-strand conformation polymorphism confirmed direct DNA sequencing. RESULTS The overall frequency was 22% (60 272 specimens), 13 60 located exons 4 or 10. A similar proportion had alterations gene (55 specimens; 22.3%) compared tumors who metastases diagnosis (5 20%; P = 0.96). Patients missense older nonsense a wild type (P 0.01). Examination revealed status concordant between biopsy later all patients. CONCLUSIONS The mutation did not differentiate presented osteosarcoma those current data indicate late events progression, because they evident before metastases. inclusion 10 increased sensitivity analysis. Cancer 2001;92:2181–9. © 2001 American Society.