Basic Fibroblast Growth Factor Synthesis by Human Peritoneal Mesothelial Cells : Induction by Interleukin-1

摘要: Peritoneal mesothelial cells are uniquely located to regulate cellular events in the peritoneal cavity and an important source for various cytokines growth factors. This study was conducted analyze capacity of human (HPMCs) synthesize release basic fibroblast factor (bFGF) characterize its regulation by inflammatory cytokines. HPMCs constitutively synthesized released considerable amounts bFGF as detected a specific immunoassay. Almost 80% (1547 +/- 173 pg/10(5) cells) localized intracellularly. Approximately 20% (357 27 associated with extracellular matrix components on HPMC surface. Small (<1%) were detectable tissue culture supernatants (8.4 1.4 cells). Treatment interleukin-1beta (IL-1beta; 1 ng/ml) resulted significant increase production. The intracellular content showed rapid but only transient increase, which above background levels after 24 hours (41% increase; P < 0.05). concentration induction bFGF. Within 96 hours, cell surface into supernatant increased 58% (564 52.4 cells; 0.01) 214% (26.4 3.2 0.001), respectively. Neither tumor necrosis factor-alpha nor interferon-gamma affected synthesis HPMCs. Stimulation IL-1beta steady-state bFGF-specific mRNA. Immunohistochemical analyses revealed constitutive expression situ proved be most pronounced areas serosal inflammation activated Our demonstrates that this is significantly up-regulated proinflammatory cytokine IL-1beta. data support view key regulators abdominal disease processes such peritonitis, fibrosis, or metastasis.