Sex identification of polar bears from blood and tissue samples
作者： S. C. Amstrup , G. W. Garner , M. A. Cronin , J. C. Patton
DOI: 10.1139/Z93-305
关键词: Ursus maritimus 、 Biology 、 Population 、 Reproduction 、 HaeIII 、 X chromosome 、 Polymerase chain reaction 、 Sexing 、 Y chromosome 、 Genetics
摘要: Polar bears (Ursus maritimus) can be adversely affected by hunting and other human perturbations because of low population densities reproduction rates. The sustainable take adult females may as 1.5% the population. Females accompanying young are most vulnerable to hunting, hunters have not consistently reported sex composition harvest, therefore a method confirm sexes polar harvested in Alaska is needed. Evidence animals often available, but blood or tissue samples are. We extracted DNA from samples, amplified segments zinc finger (ZFX ZFY) genes both X Y chromosomes with polymerase chain reaction. Digestion portions chromosome restriction enzyme HaeIII resulted subdivision original segment into four smaller fragments. did subdivide chromosome. Th...
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