Cytoplasmic accumulation of p53 protein: an independent prognostic indicator in colorectal adenocarcinomas.

摘要: BACKGROUND Aberrations of the p53 gene (also known as TP53) frequently lead to synthesis mutant proteins that accumulate in nuclei and/or cytoplasm neoplastic cells. Intracellular protein accumulation may be an unfavorable prognostic parameter breast, lung, ovarian, gastric, and colorectal cancers. Specific classes mutations, assayed by characteristic subcellular patterns, useful indicators. PURPOSE The value nuclear cytoplasmic tumor cells patients with carcinoma was studied. METHODS Antibodies PAb 1801 CM1 were used for immunocytochemical assay a retrospective series samples obtained from 206 who followed at least 5 years. Results correlated following clinicopathologic parameters: patient sex age; site, stage, grade; DNA ploidy status tumors. Overall survival disease-free analyzed Kaplan-Meier method. Differences distributions using Mantel-Cox Multivariate analysis performed Cox proportional hazards model. RESULTS Immunostaining revealed 46% (95) cases, whereas immunostaining 197 cases showed 33% (65 cases) 50% (99 respectively. In univariate analysis, both p53PAb p53CM1 accumulations significantly associated poor overall (P = .0198 P .0017, respectively) .004 .0016, respectively). When according site their tumors, statistically significant only right colon .027), left rectum .0016). multivariate .006 .002, With addition status, however, remained .035). tumors rectum, most indicator .007) .002) after disease stage. CONCLUSION Cytoplasmic accumulation, but not is independent carcinomas. IMPLICATIONS high risk recurrence benefit aggressive adjuvant therapy.