Immunohistochemical study of DNA topoisomerase I, DNA topoisomerase IIα, p53, and Ki-67 in oral preneoplastic lesions and oral squamous cell carcinomas ☆

摘要: Human DNA topoisomerase I (topo I) is the molecular target of camptothecin group anticancer drugs. Laboratory studies have shown that cellular response to topo I-targeted drugs depends on expression and replication rate apoptotic pathway activity. In this study, we tested potential indicators sensitivity in 36 cases oral squamous cell carcinoma (OSCC). Formalin-fixed, paraffin-embedded tissue sections were immunostained with monoclonal antibodies against Ki-67, p53, I, polyclonal II-alpha II-α). These markers also 18 epithelial hyperplastic lesions mild dysplasias. Immunostaining was quantified by percentage stained nuclei each sample (the labeling index); 200 immunoreactive counted per case for antibody. The results support possibility using II-α staining assessing proliferative High OSCCs suggests they may serve as inhibitors. However, assessed p53 immunostaining found be uninformative. Analysis relationship between immunohistochemical clinical pathologic parameters T N stages differentiation) showed only differentiation parameter correlated rate. Thus, significant increase poorly differentiated their higher drug treatment.