Estimate of human gene number provided by genome-wide analysis using Tetraodon nigroviridis DNA sequence
作者： Philippe Brottier , Francis Quétier , William Saurin , Jean Weissenbach , Hugues Roest Crollius
DOI: 10.1038/76118
关键词: Genome evolution 、 Genome project 、 Gene density 、 Genome 、 Biology 、 Computational biology 、 ENCODE 、 Genetics 、 Comparative genomics 、 Tetraodon nigroviridis 、 Reference genome
摘要: The number of genes in the human genome is unknown, with estimates ranging from 50,000 to 90,000 (refs 1, 2), and more than 140,000 according unpublished sources. We have developed 'Exofish', a procedure based on homology searches, identify quickly reliably. This method relies sequence another vertebrate, pufferfish Tetraodon nigroviridis, detect conserved sequences very low background. Similar Fugu rubripes, marine proposed by Brenner et al. as model for genomic studies, T. nigroviridis practical alternative also eight times compact that human. Many comparisons been made between F. rubripes DNA demonstrate potential comparative genomics using genome. Application Exofish December version working draft Unigene showed contains 28,000-34,000 genes, less 40% protein-coding fraction
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