Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)
作者： Gabriel C. Uguru , Madhav Mondhe , Shan Goh , Andrew Hesketh , Mervyn J. Bibb
DOI: 10.1371/JOURNAL.PONE.0067509
关键词: Streptomyces coelicolor 、 Actinorhodin 、 Biochemistry 、 Antisense RNA 、 RNA silencing 、 Genetics 、 Biology 、 Streptomyces 、 RNA 、 Gene expression 、 RNA interference
摘要: We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense successfully inhibited actinorhodin production. Synthetic silencing was target-specific is a new tool for regulation metabolic engineering studies Streptomyces.
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