Evidence for new forms of cardiac myosin heavy chains in mechanical heart overloading and in ageing.
作者： Catherine KLOTZ , Bernard SWYNGHEDAUW , Helene MENDES , Franicoise MAROTTE , Jean J. LEGER
DOI: 10.1111/J.1432-1033.1981.TB05253.X
关键词: Denaturation (biochemistry) 、 MYH7 、 Peptide 、 Chymotrypsin 、 Biochemistry 、 Pronase 、 Chemistry 、 Cyanogen bromide 、 Myosin 、 Sodium dodecyl sulfate 、 Biophysics
摘要: Heavy chains of myosin rods and subfragment 1 were isolated from normal hearts mechanically overloaded young older rats. These heavy-chain fragments cleaved by cyanogen bromide or partially proteolysed pronase chymotrypsin after denaturation with sodium dodecyl sulfate. The peptides, analyzed electrophoresis on a one-dimensional polyacrylamide slab gel, varied depending the origin cardiac heavy chains. Some bands present in peptide patterns heart rats missing pattern greatly hypertrophied vice versa. We conclude that mechanical overloading stimulates synthesis ‘isozyme’ primary structure which is differen: observed rat. The peptides different those for rat hearts; these results also indicate presence new heavy, chain specific to ageing. No difference was detected between rats,
sci-hub.se 参考文章(31)
J H Rader, I L Flink, E Morkin, Thyroid hormone stimulates synthesis of a cardiac myosin isozyme. Comparison of the two-two-dimensional electrophoretic patterns of the cyanogen bromide peptides of cardiac myosin heavy chains from euthyroid and thyrotoxic rabbits. Journal of Biological Chemistry. ,vol. 254, pp. 3105- 3110 ,(1979) , 10.1016/S0021-9258(17)30188-6
Ralph D Tanz, Factors influencing myocardial contractility Academic Press. ,(1967)
P Dreizen, R F Siemankowski, Canine cardiac myosin with special reference to pressure overload cardiac hypertrophy. II. Myosin ATPase. Journal of Biological Chemistry. ,vol. 253, pp. 8659- 8665 ,(1978) , 10.1016/S0021-9258(17)34341-7
A G Weeds, R S Taylor, The magnesium-ion-dependent adenosine triphosphatase of bovine cardiac Myosin and its subfragment-1 Biochemical Journal. ,vol. 159, pp. 301- 315 ,(1976) , 10.1042/BJ1590301
L. S. Malchikova, G. B. Asafoc, E. I. Chazov, N. V. Lipina, V. N. Smirnov, Taurine and Electrical Activity of the Heart Circulation Research. ,vol. 35, pp. 11- 21 ,(1974) , 10.1161/01.RES.35.3_SUPPL.III-11
Anne-Marie Lompre, Ketty Schwartz, Anne d'Albis, Gabrielle Lacombe, Nguyen Van Thiem, Bernard Swynghedauw, Myosin isoenzyme redistribution in chronic heart overload Nature. ,vol. 282, pp. 105- 107 ,(1979) , 10.1038/282105A0
CHARLES K. FRIEDBERG, RICHARD P. LASSER, DOUGLAS F. ALLEN, STEPHEN E. FURST, GEORGE E. GABOR, Production of Chronic Elevation of Left Ventricular End Diastolic Pressure in Dogs: Hemodynamic and Renal Studies Circulation Research. ,vol. 15, pp. 1- 10 ,(1964) , 10.1161/01.RES.15.1.1
M.A. Porzio, A.M. Pearson, Improved resolution of myofibrillar proteins with sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Biochimica et Biophysica Acta. ,vol. 490, pp. 27- 34 ,(1977) , 10.1016/0005-2795(77)90102-7
F Meerson, Decrease in the rate of RNA and protein synthesis and degradation in the myocardium under long-term compensatory hyperfunction and on aging Journal of Molecular and Cellular Cardiology. ,vol. 10, pp. 145- 159 ,(1978) , 10.1016/0022-2828(78)90039-1
Raye Z. Litten, Joseph E. Brayden, Norman R. Alpert, The ATPase activity of subfragment-1 from the hypertrophied heart Biochimica et Biophysica Acta (BBA) - Enzymology. ,vol. 523, pp. 377- 384 ,(1978) , 10.1016/0005-2744(78)90040-2