Species correlations between cardiac isomyosins. A comparison of electrophoretic and immunological properties.

摘要: Structural relationships between cardiac isomyosins were analyzed in 10 species using native-gel electrophoresis and radioimmunoassay. In the rat rabbit, three types of ventricular isomyosin, V1, V2, V3, identified by electrophoresis. Monoclonal antibodies specific for heavy chains either type V1 or V3 isomyosin rabbit used comparison immunological atrial myosins other species. Normal guinea pig myosin reacted with both anti-V2 anti-V3 antibodies, but only a single band was detected this When thyrotoxic hypertrophy induced pigs, there decrease reactivity antibody an increase anti-V1 reactivity. This change indicated proportions two ventricle even though no heterogeneity six including Xenopus, chicken, dog, pig, beef, human, electrophoresis, each antibody. mouse, also However, unlike mouse equally antibodies. conclusion, we have highly conserved epitopes myosin, which found to specifically occur on high Ca2+-ATPase lower ATPase most examined.