Biotransformation of p-methoxyphenylacetonitrile into p-methoxyphenylacetic acid by resting cells of Bacillus subtilis.

摘要: Resting cells of Bacillus subtilis ZJB-063 were used for the direct transformation MOPAN (p-methoxyphenylacetonitrile) to MOPAA (p-methoxyphenylacetic acid), which is an important pharmaceutical intermediate. The B. culture conditions production nitrilase and reaction this nitrilase-mediated conversion optimized. maximum was achieved when glucose a combination ammonium sulfate yeast powder added as carbon nitrogen sources respectively. Previously reported inducers found be unnecessary from ZJB-063, indicated that appeared constitutive. However, epsilon-caprolactam (6-hexanolactam) inducer, exhibited nitrile hydratase amidase activity. into (specific activity 17.03 units.g(-1)(DCW); DCW dry cell weight) observed in solution containing 50 mM phosphate buffer (pH 7.0), 10 MOPAN, 2.7 mg DCW.ml(-1) wet resting 5% (v/v) DMSO 4 h at 32 degrees C. (10 mM) completely converted (9.65 5 shake flasks without formation p-methoxyphenylacetamide. small deviation theoretical amount may due partial consumption products by ZJB-063. Both inhibited hydrolysis concentrations above 15 mM. Scale up 200 ml bubble bioreactor shortened time compared with reactions performed flasks.