A simple method for coupling proteins to insoluble polysaccharides.
作者： C. J. Sanderson , D. V. Wilson
DOI:
关键词: Sodium borohydride 、 Side chain 、 Coupling (electronics) 、 Periodate 、 Chemistry 、 Polymer chemistry 、 Aldehyde 、 Polysaccharide 、 Biochemistry
摘要: Proteins have been coupled to insoluble polysaccharides following periodate oxidation produce reactive aldehyde groups which will react with side chain amino of proteins. The coupling is stabilized and unreacted aldehydes blocked, by reduction sodium borohydride.
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