A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum
作者： Nathan C Shaner , Gerard G Lambert , Andrew Chammas , Yuhui Ni , Paula J Cranfill
DOI: 10.1038/NMETH.2413
关键词: Fluorescence 、 Bimolecular fluorescence complementation 、 Molecular biology 、 Green fluorescent protein 、 Förster resonance energy transfer 、 Fluorescence-lifetime imaging microscopy 、 Biophysics 、 Yellow fluorescent protein 、 Branchiostoma lanceolatum 、 Cyan 、 Biology 、 Biotechnology 、 Cell biology 、 Biochemistry
摘要: We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins.
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