Superresolution live imaging of plant cells using structured illumination microscopy

摘要: This protocol describes a detailed method for superresolution imaging of plant tissues by structured illumination microscopy (SIM). Details include microscope calibration, tissue preparation, image acquisition and evaluation SIM images. Although (SR) approaches have been routinely used fixed or living material from other organisms, the use time-lapse (SIM) in cells still remains under-developed. Here we describe validated that focuses on cortical microtubules different cell types. By using one existing commercially available platforms, provide user-friendly easy-to-follow may be widely applied to cells. includes steps describing calibration channel alignment, generation an experimental point spread function (PSF), preparation appropriate observation chambers with material, acquisition, reconstruction validation. can carried out within two three working days.