Application of the MAST Immunodiagnostic System to the determination of allergen-specific IgE.

作者: S P Miller , V A Marinkovich , D H Riege , W J Sell , D L Baker

DOI: 10.1093/CLINCHEM/30.9.1467

关键词: AllergyMast (sailing)ImmunologyTest chamberAllergenAllergen specific IgEImmunoglobulin EAllergy testingAntibodyComputer science

摘要: The MAST Immunodiagnostic Test System was developed to provide a comprehensive, simple means for the in vitro measurement of multiple antigens or antibodies. first commercial application system incorporates several novel features cost-effective diagnosis IgE-mediated allergy clinical laboratory physician's office. basis is unique analytical test chamber, which contains cellulose thread as solid-phase matrix and allows results from single assay. This chamber both positive negative controls requires no volume-dependent pipetting steps. Immunographic exposure onto high-speed Polaroid instant film quantifying with an automatic recording infrared-transmittance densitometer. are easily interpreted by using patient record provided system. greatly simplifies testing allergen-specific IgE, while retaining specificity sensitivity. Currently, one can simultaneously measure picomoles IgE up 35 different allergen classes. In addition testing, technology applicable other immunodiagnostic profiles.

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