The use of patterned dual thermoresponsive surfaces for the collective recovery as co-cultured cell sheets

摘要: Heterotypic cell interactions are critical to achieve and maintain specific functions in many tissues organs. We have focused on patterned structure surfaces enable co-culture of heterotypic cells recovery co-cultured sheets for applications tissue engineering. Thermoresponsive polymers exhibiting different transition temperatures water comprise both poly(N-isopropylacrylamide) (PIPAAm) n-butyl methacrylate (BMA) co-grafted as side chains PIPAAm main chains. These copolymers were surface-grafted patterns obtain dual thermoresponsive culture using electron beam polymerisation method porous metal masks. On surfaces, site-selective adhesion growth rat primary hepatocytes (HCs) bovine carotid endothelial (ECs) allowed co-culture, exploiting hydrophobic/hydrophilic surface chemistry regulated by temperature the sole variable. At 27°C, seeded HCs adhered exclusively onto hydrophobic, dehydrated P(IPAAm–BMA) domains (1-mm∅ area), but not neighbouring hydrated domains. Sequentially ECs then hydrophobised upon increasing 37°C, achieving co-cultures. Reducing 20°C promoted hydration polymer-grafted domains, permitting release co-cultured, monolayers continuous with interactions. Recovered can be manipulated, moved sandwiched other structures, providing new useful constructs basic biology research preparation tissue-mimicking multi-layer materials through overlaying sheets.