A Novel β-adaptin/c-Myc Complex Formation Modulated by Oxidative Stress in the Control of the Cell Cycle in Macrophages and its Implication in Atherogenesis

摘要: Our study tested the proposal that c-Myc activation in macrophages is differentially carried out dependent on intracellular oxidative state of cells and potentially associated to process atherogenesis. Under our experimental conditions, generation reactive oxygen species by presence oxidized low density lipoproteins (oxLDL) or Gram negative bacterial lipopolysaccharides (LPS) modifies expression cellular adhesion molecules such as c-Abl, calcium transport proteins plasma membrane Ca2+-ATPase (PMCA), CD47, procaspase-7, CASP7, CHOP, transcriptional activators c-Jun participate endocytosis like α- β-adaptin. We present first evidence showing a stress alters c-Myc-dependent activity pathways through binding β-adaptin promoting reversible formation complex presents ability regulate development cell cycle. propose subtle regulation this c-Myc/β-adaptin when change from normal physiological condition stress, represents defense mechanism against deleterious effects caused loss homeostasis.