Inhibition of phosphatidic acid phosphohydrolase activity by sphingosine
作者： Yaakov Lavie , Orly Piterman , Mordechai Liscovitch
DOI: 10.1016/0014-5793(90)80796-L
关键词: Phosphatidic acid 、 Sphingosine 、 Phospholipase D activity 、 Phosphatidylcholine 、 Phospholipase D 、 Biology 、 Diacylglycerol kinase 、 Second messenger system 、 Biochemistry 、 Phospholipid 、 Biophysics 、 Genetics 、 Cell biology 、 Molecular biology 、 Structural biology
摘要: Recent evidence indicates that a major fraction of diacylglycerol is produced in hormonally stimulated cells arises by phosphatidylcholine hydrolysis via the sequential action phospholipase D and phosphatidic acid phosphohydrolase (PAP). We have previously reported sphingoid bases stimulate activity NG108-15 cells. The presented here demonstrates sphingosine-treated cells, elevated levels are accompanied parallel, time- dose-dependent decrease levels. DL-propranolol, known inhibitor PAP, exerted similar effects, suggesting sphingosine may been due to inhibition PAP activity. This prediction was confirmed vitro experiments which it demonstrated as potent an both cytosolic membrane-associated propranolol. hypothesis exert dual signal termination proposed.
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