Reduction in Inflammatory Gene Expression in Skeletal Muscle from Roux-en-Y Gastric Bypass Patients Randomized to Omentectomy

摘要: Objectives To examine the effects of Roux-en-Y gastric bypass (RYGB) surgery with and without laparoscopic removal omental fat (omentectomy) on temporal gene expression profiles skeletal muscle. Design Previously reported were whole-body metabolic a randomized, single-blinded study in patients receiving RYGB stratified to receive or not omentectomy. In this follow up we report changes muscle subset 21 patients, for whom biopsies collected preoperatively at either 6 months 12 postoperatively. Methodology/Principal Findings RNA isolated from subjects (8 omentectomy 13 omentectomy) taken before postoperatively subjected profiling via Exon 1.0 S/T Array Taqman Low Density Array. Robust Multichip Analysis enrichment data analysis revealed 84 genes least 4-fold difference after surgery. At group displayed greater reduction associated inflammation (ANKRD1, CDR1, CH25H, CXCL2, CX3CR1, IL8, LBP, NFIL3, SELE, SOCS3, TNFAIP3, ZFP36) relative non-omentectomy group. Expressions IL6 CCL2 decreased all postoperative time points. There was differential driving protein turnover (IGFN1, FBXW10) both groups over increased PAAF1 months. Evidence activation satellite cells inferred up-regulation HOXC10. The elevated post-operative 22 small nucleolar RNAs transcription factors JUNB, FOS, FOSB, ATF3 MYC, EGR1 as well orphan nuclear receptors NR4A1, NR4A2, NR4A3 suggest dramatic reorganizations cellular genetic levels. Conclusions/Significance These indicate that reduces inflammation, further amplifies response. Trial Registration ClinicalTrials.gov NCT00212160