Architectural protein Pita cooperates with dCTCF in organization of functional boundaries in Bithorax complex
作者： Olga Kyrchanova , Nikolay Zolotarev , Vladic Mogila , Oksana Maksimenko , Paul Schedl
DOI: 10.1242/DEV.149815
关键词: Biology 、 Crosstalk (biology) 、 Cell biology 、 Insulator (genetics) 、 Protein–protein interaction 、 Genetics 、 Bithorax complex 、 Homeotic gene 、 Mutant
摘要: Boundaries in the Bithorax complex (BX-C) of Drosophila delimit autonomous regulatory domains that drive parasegment-specific expression homeotic genes. BX-C boundaries have two crucial functions: they must block crosstalk between adjacent and at same time facilitate boundary bypass. The C2H2 zinc-finger protein Pita binds to several boundaries, including Fab-7 Mcp To study functions, we used a replacement strategy by substituting modified DNAs for boundary, which is located iab-6 iab-7 domains. Multimerized sites iab-6↔iab-7 but fail support regulation Abd-B (bypass). In case Fab-7, novel sensitized background show Pita-binding contribute its function. Although from BX-C, it does not function appropriately when substituted Fab-7: blocks Mutation site disrupts blocking activity also eliminates dCTCF binding. contrast, mutation affect binding, this mutant retains partial
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