Effect of Staining and Freezing Media on Sortability of Stallion Spermatozoa and their Post-thaw Viability After Sex-sorting and Cryopreservation
作者： JR Clulow , H Buss , G Evans , H Sieme , D Rath
DOI: 10.1111/J.1439-0531.2007.01010.X
关键词: Sperm 、 Biology 、 Sperm motility 、 Sex sorting 、 Insemination 、 Andrology 、 Staining 、 Incubation 、 Cryopreservation 、 Coloring agents
摘要: Sex-sorted, frozen-thawed stallion spermatozoa remain out of reach commercial horse breeders because the low efficiency sex-sorting process and unacceptable fertility rates after insemination. Two experiments were designed to test effects alternative staining freezing media improve viability sex-sorted spermatozoa. Experiment 1 compared two media, INRA 82(®) a modified lactose-ethylenediaminetetraacetic acid (EDTA), for cryopreservation No significant differences between could be identified, suggesting that both cryodiluents would suitable incorporation into sex-preselection protocol 2 Kenney's Tyrode's (KMT) Sperm TALP (Sp-TALP) as incubation medium prior sex-sorting. A increase in percentage acrosome-reacted occurred clarified Sp-TALP with KMT. As no improvements sorting achieved using Sp-TALP, it was concluded protocols include KMT while either or lactose-EDTA employed cryodiluents.
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