Corticotropin-releasing factor receptor expression in the pituitary of fetal sheep after lesion of the hypothalamic paraventricular nucleus.

摘要: Both the capacity of CRF to release ACTH and number binding sites for in anterior pituitary decline during final weeks gestation fetal sheep. The present study examined regulation receptor expression by hypothalamic paraventricular nucleus (PVN) late Bilateral radiofrequency lesions PVN (PVN-Lx; n = 4) or sham (SHAM; 5) were performed sheep at 118–122 days gestational age (dGA). Pituitary glands from PVN-Lx SHAM fetuses collected 139–142 dGA (term, approximately 148 dGA). Dual-label situ hybridization was using a digoxigenin-labeled ovine POMC complementary RNA, together with 35S-labeled type I (CRF1) localize quantify CRF1 mRNA POMC-hybridizing cells. Binding of[ 125I]-ovine also both autoradiography. signal ...