PCR amplification of 16S rDNA sequences in Fe-rich sediment of coal refuse drainage
作者： Yueli Hao , Warren A. Dick , Olli H. Tuovinen
关键词: Bacteria 、 Ribosomal DNA 、 Coal mining 、 Sediment 、 16S ribosomal RNA 、 Chemistry 、 Coal 、 Pyrite 、 Acid mine drainage 、 Mineralogy
摘要: Acid drainage from coal mines or refuse, when treated in collection ponds, results the formation of Fe-rich sediments. The Fe these sediments inhibits PCR and hinders identification bacteria using non-culture 16S rDNA molecular methods. We describe a technique that employs EDTA to dissolve remove sediments, yielding DNA can be amplified by PCR. allows bacterial sequences increases our understanding community
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