Evaluation of Bacterial Community Structure and Its Influence on Sulfide Oxidation in a Bio-Leaching Environment
作者： Mazda Biglari , Jeno M. Scharer , Ronald V. Nicholson , Trevor C. Charles
DOI: 10.1080/01490450802602716
关键词: Inorganic chemistry 、 Sulfide 、 Nuclear chemistry 、 Iron bacteria 、 Bacteria 、 Specific surface area 、 Pyrite 、 Acidithiobacillus 、 Leaching (metallurgy) 、 Sulfur 、 Chemistry
摘要: The mechanism of sulfide oxidation by adhering bacteria (direct mechanism) and ferric ion in the aqueous phase was studied quantitative assessment bacterial activity on surface. To probe for principal species surface supernatant, a library DNA genes encoding portions 16S rRNA constructed. PCR-amplified from populations cloned employing PROMEGA's pGEM-T Easy Vector system. clone frequency indicated that iron-oxidizing were dominant liquid phase, while Acidithiobacillus ferroixdans, which is both sulfur iron oxidizer most prevalent Samples crystalline pyrite exposed to consortium evaluate alterations caused bacteria. Chemical (abiotic) experiments with as oxidant carried out parallel biological tests. Changes topography monitored atomic...
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