PCR-mediated detection of acidophilic, bioleaching-associated bacteria.
作者： P De Wulf-Durand , L J Bryant , L I Sly
DOI: 10.1128/AEM.63.7.2944-2948.1997
关键词:
摘要: The detection of acidophilic microorganisms from mining environments by culture methods is time consuming and unreliable. Several PCR approaches were developed to amplify small-subunit rRNA sequences the DNA six bacterial phylotypes associated with acidic environments, permitting target at concentrations as low 10 fg.
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