Efficient nonviral transfection of dendritic cells and their use for in vivo immunization.

摘要: Immunization with dendritic cells (DCs) transfected genes encoding tumor-associated antigens (TAAs) is a highly promising approach to cancer immunotherapy. We have developed system, using complexes of plasmid DNA expression constructs the cationic peptide CL22, that transfects human monocyte-derived DCs much more efficiently than alternative nonviral agents. After CL22 transfection, expressing stimulated autologous T in vitro and elicited primary immune responses syngeneic mice, an antigen-specific manner. Injection CL22-transfected TAA, but not pulsed TAA-derived peptide, protected mice from lethal challenge tumor aggressive model melanoma. The system fast efficient viral vectors for engineering use immunotherapy research.