Probing Dendritic Cell Function by Guiding the Differentiation of Embryonic Stem Cells

摘要: Publisher Summary Dendritic cells (DC) are unique among the populations of antigen presenting by virtue their capacity to direct outcome recognition naive T cells. By serving as messengers from site infection, DC inspect cell repertoire, identifying those specific for cargo antigens they display on surface peptide-MHC complexes. Having encountered with a complementary receptor antigen, deliver instructions directing expansion relevant clones and deployment front line immune response. Should these pose threat integrity host, may decommission them active service, thereby minimizing any damage friendly fire. The role played in fine-tuning opposing forces self-tolerance immunity makes attractive candidates intervention variety disease states. Alternatively, specifically enhancing immunogenicity, might be coerced into breaking natural state tumor-associated (TAA) full force response towards transformed established tumors. Despite clinical potential, very little is known molecular basis function, which permits fulfill conflicting roles. An approach has been developed study DC, draws features embryonic stem (ES) cells: self-renewal, pluripotency, tractability genetic modification. This chapter describes detail protocols involved discusses advantages such an experimental system provides over current art.