AN EVOLUTIONARY CONSERVED COUP-TF BINDING ELEMENT IN A NEURAL-SPECIFIC GENE AND COUP-TF EXPRESSION PATTERNS SUPPORT A MAJOR ROLE FOR COUP-TF IN NEURAL DEVELOPMENT

摘要: The COUP transcription factors (COUP-TF and ARP-1) are the most highly conserved members of nuclear receptor superfamily throughout evolution. Previous studies indicated that orphan receptors may be involved in early neurogenesis Drosophila zebrafish. Here we identified a neural-specific gene, arrestin, whose can regulated by endogenous COUPs through DR-7 element (direct repeat with 7-base pair spacer) located upstream start site. Importantly, binding site arrestin gene promoter is among mouse, bovine, human. However, mouse also capable responding to retinoic acid while human does not. Expression COUP-TF correlates known expression sites vivo, notably during differentiation retina. We show expressed spatio-temporally defined pattern murine central nervous system embryogenesis. It appears that...