Idala: An unnamed Function Peptide Vaccine for Tuberculosis
作者： S Ismail , NT Darwish , TC Teoh , MA Abdulla , R Omar
DOI: 10.4314/TJPR.V11I6.2
关键词: Biology 、 Peptide vaccine 、 Immunogenicity 、 Vaccination 、 Recombinant DNA 、 Virology 、 Antibody 、 Expression vector 、 Escherichia coli 、 Antigenicity
摘要: Purpose: To evaluate Myt272 protein antigenicity and immunogenicity by trial vaccination in mice its silico analysis as a potential peptide vaccine for tuberculosis. Methods: gene, which has 100 % identity with Mycobacterium tuberculosis H37Rv unknown function gene Rv3424c, was ligated genomic shotgun approach into the expression vector pQE32, transformed Escherichia coli SG13009. Expression during cell growth induced isopropyl-β-D-thiogalactopyranoside. The recombinant isolated from harvested lysate injected experiment up to 42 days. ELISA tests anti-His antibodies were performed on collected individual blood samples’ sera. Color development microplate reader measured at 450 nm. Results: predicted have mass of approximately 13 kDa present soluble fraction lysate. test revealed very statistically significant high levels detected sera immunized group compared negative controls. Conclusion: A 10.1 kD unnamed (IDALA) Rv3424 could be tested experiment.
utoronto.ca
tjpr.org 参考文章(21)
Hans J. Hedrich, The laboratory mouse Elsevier Academic Press. ,(2012)
Sangita Mukhopadhyay, Kithiganahalli Narayanaswamy Balaji, The PE and PPE proteins of Mycobacterium tuberculosis Tuberculosis. ,vol. 91, pp. 441- 447 ,(2011) , 10.1016/J.TUBE.2011.04.004
Ian M. Orme, Preclinical testing of new vaccines for tuberculosis: a comprehensive review. Vaccine. ,vol. 24, pp. 2- 19 ,(2006) , 10.1016/J.VACCINE.2005.07.078
Buka Samten, Xisheng Wang, Peter F. Barnes, Immune regulatory activities of early secreted antigenic target of 6-kD protein of Mycobacterium tuberculosis and implications for tuberculosis vaccine design. Tuberculosis. ,vol. 91, ,(2011) , 10.1016/J.TUBE.2011.10.020
S. N. Cohen, A. C. Y. Chang, H. W. Boyer, R. B. Helling, Construction of Biologically Functional Bacterial Plasmids In Vitro Proceedings of the National Academy of Sciences of the United States of America. ,vol. 70, pp. 3240- 3244 ,(1973) , 10.1073/PNAS.70.11.3240
E. Hochuli, W. Bannwarth, H. Döbeli, R. Gentz, D. Stüber, Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent Nature Biotechnology. ,vol. 6, pp. 1321- 1325 ,(1988) , 10.1038/NBT1188-1321
E. P. v. Strandmann, C. Zoidl, H. Nakhei, B. Holewa, R. P. v. Strandmann, P. Lorenz, L. Klein-Hitpass, G. U. Ryffel, A highly specific and sensitive monoclonal antibody detecting histidine-tagged recombinant proteins Protein Engineering. ,vol. 8, pp. 733- 735 ,(1995) , 10.1093/PROTEIN/8.7.733
Leif Wide, Jerker Porath, Radioimmunoassay of proteins with the use of Sephadex-coupled antibodies Biochimica et Biophysica Acta. ,vol. 130, pp. 257- 260 ,(1966) , 10.1016/0304-4165(66)90032-8
R. Janknecht, G. de Martynoff, J. Lou, R. A. Hipskind, A. Nordheim, H. G. Stunnenberg, Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 8972- 8976 ,(1991) , 10.1073/PNAS.88.20.8972
Abul K Azad, Tatiana D Sirakova, Norvin D Fernandes, Pappachan E Kolattukudy, None, Gene Knockout Reveals a Novel Gene Cluster for the Synthesis of a Class of Cell Wall Lipids Unique to Pathogenic Mycobacteria Journal of Biological Chemistry. ,vol. 272, pp. 16741- 16745 ,(1997) , 10.1074/JBC.272.27.16741