Archaic Strains of Methicillin-Resistant Staphylococcus aureus: Molecular and Microbiological Properties of Isolates from the 1960s in Denmark
作者： HERMÍNIA DE LENCASTRE , MARILYN CHUNG , HENRIK WESTH
DOI: 10.1089/MDR.2000.6.1
关键词: Bacteria 、 Microbiology 、 Lineage (evolution) 、 Gel electrophoresis 、 Isolation (microbiology) 、 Staphylococcus aureus 、 Pulsed-field gel electrophoresis 、 Regulator gene 、 Methicillin-resistant Staphylococcus aureus 、 Biology
摘要: ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) isolated in the 1960s Denmark are among historically earliest samples of these bacteria. We determined microbiological and molecular properties 46 such isolates. They showed remarkably uniform properties, which included: (i) low methicillin MIC value (6–25 μg/ml); (ii) heterogeneous expression resistance; (iii) presence a single, common, mecA polymorph II; (iv) lack regulatory gene mecI; (v) frequent Tn554; (vi) common pulsed-field gel electrophoresis (PFGE) type. These together with chronological dates isolation, recovery strains from 18 hospitals scattered over Denmark, suggest that they represent lineage close time to evolutionary origin European MRSA.
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