CD81 is required for rhoptry discharge during host cell invasion by Plasmodium yoelii sporozoites.

摘要: Summary Plasmodium sporozoites are transmitted by Anopheles mosquitoes and first infect the liver of their mammalian host, where they develop as stages before onset erythrocytic infection malaria symptoms. Sporozoite entry into hepatocytes is an attractive target for anti-malarial prophylactic strategies but remains poorly understood at molecular level. Apicomplexan parasites invade host cells forming a parasitophorous vacuole that essential parasite development, process involves secretion apical organelles called rhoptries. We previously reported membrane protein CD81 required Plasmodium falciparum yoelii sporozoites. acts early stage infection, possibly step, mechanisms involved still unknown. To investigate role during sporozoite entry, we generated transgenic P. yoelii expressing fluorescent versions three known rhoptry proteins, RON2, RON4 RAP2/3. observed RON2 lost following discharge merozoite entry. In contrast, our data indicate RAP2/3 secreted infection. further show occurs only in presence CD81, providing direct evidence productive invasion.